pabA [2019-08-15 13:43:47]

para-aminobenzoate synthase (subunit B)/ anthranilate synthase (subunit II)

Locus: BSU_00750

Isoelectric point: 4.78

Molecular weight: 21.54 kDa

Protein length: 194 aa

Gene length: 585 bp

Function: biosynthesis of folate and tryptophan

Product: para-aminobenzoate synthase (subunit B)/ anthranilate synthase (subunit II) glutamine amidotransferase (subunit B) and anthranilate synthase (subunit II)

Essential: no

E.C.: 2.6.1.85

Synonyms: trpG,trpX

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of aromatic amino acids
• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis of folate

Gene

Coordinates: 84,290 84,874

The protein

Catalyzed reaction/ biological activity

• Chorismate + L-glutamine --> anthranilate + pyruvate + L-glutamate (according to UniProt)

Domains

• Glutamine amidotransferase type-1 domain (aa 1-194) (according to UniProt)

Structure

• 1I1Q (from Salmonella typhimurium, 42% identity) PubMed

Expression and Regulation

Operons

• Genes: pabB-pabA-pabC-sul-folB-folK-yazB-yacF-lysS
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulation

  • translation repressed by tryptophan (MtrB) PubMed

Other regulations

• MtrB: translation inhibition, PubMed

Additional information

• the pabA-pabC part of the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• BKE00750 (pabA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTAAAATCATTTCTCCGC, downstream forward: _UP4_TATCGCAAGGAAGTTATTGC
• BKK00750 (pabA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTAAAATCATTTCTCCGC, downstream forward: _UP4_TATCGCAAGGAAGTTATTGC

References

Reviews

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Original Publications

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